Invitrogen platinum taq manual

Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the highest sensitivity without false-positive results from reagent-borne . Invitrogen ™ Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. It retains all the features of the Platinum™ SuperFi™ DNA Polymerase. ∤ Platinum ™ SuperFi DNA Polymerase is a proofreading DNA polymerase that combines fidelity with Platinum™ hot-start. Invitrogen™ Platinum™. Taq. DNA Polymerase. Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. Manufacturer: Invitrogen™ View more versions of this product. Catalog No. $4, / Each. Qty Check Availability.

RT/Platinum™ Taq Mix 1 μL — Autoclaved distilled water to 50 μL — Note: Verify the absence of genomic DNA in RNA preparations by omitting the RT/Platinum™ Taq Mix and substituting 2 units of Platinum™ Taq DNA Polymerase in the reaction. 3. Gently mix and make sure that all the components are at the bottom of the amplification tube. Fidelity vs. Taq: X Format: Master Mix PCR setup Component µL rxn µL rxn Custom Final conc. Water, nuclease-free to 25 μL to 50 μL to μL — Invitrogen™ Platinum. Invitrogen™ Platinum™ II Hot-Start Green PCR Master Mix (2X) Enzyme characteristics Hot-start: Antibody Length: Up to 5 kb Fidelity vs. Taq: 1X Format: Master mix PCR setup Use the measurements below to prepare your PCR experiment, or enter your own parameters in the column provided. Component µL rxn µL rxn Custom Final conc.

1. Measure out the amount of Taq DNA polymerase needed. When using a reaction cocktail, determine the total number of units in the mix. 2. Add a number of “equivalent units” of Platinum® Taq Antibody equal to the amount of Taq DNA polymerase units in the cocktail. For Taq DNA polymerase at a concentration of 5 U/μL, prepare a mixture. Mix well. Invitrogen™ ROX Reference Dye (Cat. no. ) ∤ for PCR: Invitrogen™ E-Gel™ General Purpose Gels, % (Cat. no. G) Invitrogen™ TrackIt™ 1 kb Plus DNA Ladder (Cat. no. ) Timing Varies depending on amplicon length Product description ∤ Low Glycerol Platinum™ Taq, CG DNA Polymerase is a. Platinum® Taq DNA High Fidelity Polymerase (5 U/µL) µL 1 U* * 1 U is sufficient for amplifying most targets. In some cases, more enzyme may be required (up to units). Use 1 U of enzyme for targets 12 kb. Mix and briefly centrifuge the components. 3 Add template DNA and primers.